Peptide Dosage & Reconstitution Calculator
Enter the peptide amount in the vial, how much bacteriostatic water you added, and your target dose. You get the concentration, the draw volume, and the equivalent units on a standard insulin syringe, instantly.
Photo: Stephan HK / UnsplashVial concentration ÷ desired dose = how much to draw into the syringe. That’s the whole calculation, the calculator below just does the arithmetic for you.
Three numbers, one formula.
Reconstituting a peptide just means dissolving the freeze-dried powder in a liquid so it can be measured and injected. The concentration you end up with is the peptide mass divided by however much liquid you added: a 5mg vial mixed with 2mL of water gives you 2,500 mcg per mL. Add more water and the same 5mg spreads thinner; add less and it's more concentrated.
Once you know the concentration, your draw volume for any target dose is just that dose divided by the concentration. A 250 mcg dose at 2,500 mcg/mL works out to 0.1 mL, which is 10 units on a standard 100-unit (1mL) insulin syringe. That's the entire calculation, the tool above just runs the numbers so you don't have to do the division by hand every time.
The one thing the math can't fix is measurement error at either end: how precisely the vial was actually filled, and how precisely you read the syringe markings. Smaller draw volumes are harder to measure accurately on a standard syringe, which is why a lot of people pick a dilution that lands their usual dose somewhere in the middle of the syringe's scale rather than at the very bottom.
What people commonly cite, by peptide.
Not a recommendation, just what shows up most often in non-clinical and anecdotal protocols. Full evidence and sourcing on each peptide’s own page.
BPC-157→
| What's cited | Range |
|---|---|
| Commonly cited daily range | 250-500 mcg, once or twice daily |
| Commonly cited upper range | up to 1,000 mcg/day in some non-clinical protocols |
| Commonly cited weight-based range | ~2-4 mcg per kg of bodyweight per day, cited as an alternative to a flat dose in some protocols |
| Typical cited cycle length | 4-6 weeks, often followed by a break |
| Human safety pilot (IV, n=2) | up to 20 mg single infusion, no adverse events reported |
TB-500→
| What's cited | Range |
|---|---|
| Commonly cited loading dose | 2-2.5 mg, twice weekly |
| Commonly cited loading phase length | 4-6 weeks |
| Commonly cited maintenance dose | 2-2.5 mg, once weekly |
| Commonly cited weekly total | 2-5 mg/week across sources |
AOD-9604→
| What's cited | Range |
|---|---|
| Commonly cited daily range | 250-500 mcg, once daily, subcutaneous |
| Commonly cited upper range | up to 1,000 mcg/day in some protocols |
| Phase IIb clinical trial dose (weight loss) | 1 mg/day, 12-24 weeks |
| Typical cited cycle length | 12-24 weeks |
Thymosin Alpha 1→
| What's cited | Range |
|---|---|
| Clinical dose (Zadaxin, hepatitis B, approved abroad) | 1.6 mg, subcutaneous, twice weekly, 6-12 months |
| Sepsis trial dose (ETASS RCT) | 1.6 mg, twice weekly, alongside standard care |
| Commonly cited "immune support" protocol (unofficial, US biohacking) | 300-500 mcg/day |
Calculator questions, answered.
There's no single right answer, it depends on the concentration you want. More water means a more dilute solution (easier to dose small amounts precisely, but a bigger injection volume); less water means a more concentrated solution. A common approach is to pick a water volume that lands your typical dose somewhere between 10 and 50 units on a 100-unit insulin syringe, since that range is easiest to measure accurately. Enter a few different water volumes into the calculator above to see how the draw volume changes.
It depends entirely on your concentration (vial mg ÷ water mL). The calculator above converts your specific vial size, dilution, and target dose into both milliliters and units on a standard 100-unit (1mL) insulin syringe, so you're not doing the conversion by hand.
The math itself (concentration = peptide mass ÷ diluent volume) is exact, it's just arithmetic. What it can't account for is measurement error from your syringe, inconsistencies in how a vendor's vial was actually filled, or degradation from improper storage. Treat the output as the correct math for the numbers you entered, not a guarantee about what's actually in the vial.
Bacteriostatic water is sterile water with a small amount of benzyl alcohol added, which inhibits bacterial growth so a multi-dose vial can be reused over days or weeks without needing a fresh sterile vial each time. Plain sterile water lacks that preservative and is typically intended for single use.
Yes, the math (mg in the vial, mL of diluent, target dose in mcg) is the same regardless of which peptide you're reconstituting. What's specific to each peptide is what dose is commonly cited or clinically relevant, which is covered on each peptide's own page, not by this calculator.
Now check the evidence behind the number.
The calculator gives you a number. PepGuard tells you if it's worth trusting.
Check any peptide's evidence score, FDA status, and safety signals before it goes anywhere near your body.